Biomimetic in vitro culture models for evaluation of novel bone substitute implants
Project title: Biomimetic in vitro culture models for evaluation of novel bone substitute implants
Recruiting and hosting institution: University of Belgrade, Faculty of Technology and Metallurgy
PhD enrollment: University of Belgrade, Faculty of Technology and Metallurgy
Supervisors: University of Belgrade, Faculty of Technology and Metallurgy (RS): B. Obradovic, Politecnico di Torino (IT), F. Baino.
Objectives: To develop and utilize cell and tissue culture models for physiologically relevant evaluation of bone substitute implants.
Expected Results: Establishment of in vitro models for evaluation of biomaterials by using cultures of murine preosteoblast cells and porcine bone explants (ex vivo testing) and two biomimetic bioreactors imitating physiological conditions in bone tissue.
Description: DC9 position is about investigating the following aspects
- Utilization of selected novel biomaterials anddevelopment of procedures for seeding of murine preosteoblast cells onto the samples; short- and longer-term cultivation of the obtained constructs in two biomimetic bioreactors: perfusion bioreactor and bioreactor with dynamic compression and perfusion, so to imitate the bone tissue environment.
- Utilization of selected novel biomaterials in cultures of porcine bone explants (ex vivo testing) under static as well as bioreactor conditions.
- Analysis of the effects of culture conditions (i.e. flowrate, shears stresses, dynamic compression, cell seeding density, geometry of the biomaterial-bone tissue construct) on selected biomaterials as well as cell- and bone tissue-biomaterial interactions (by determining biomaterial stability, cell distribution, viability and differentiation, morphological features of cells and explant tissue, gene expressions in cultivated cells and cytokine profiles in the culture media); validation of the developed in vitro models for preclinical physiologically relevant assessment of biomaterials by comprehensive analyses of the obtained results in comparison to those obtained in static cultures as well as to literature data on in vivo implantation of similar materials.
The Doctoral Candidates will interact with other members of the project’s DCs in a multidisciplinary setting by taking part in training sessions and workshops. Each DC will also conduct secondments in order to broaden her/his scientific understanding of the project’s subject and develop soft skills.
Required profile/research interests:
- Master’s degree in Biomedical Engineering, Materials Engineering or related fields.
- The degree must be completed with good grades, latest by the start of the recruitment.
- Proficiency in the English language (both written and spoken): CAMBRIDGE ≥ FCE grade B (or equivalent).
- Good communication skills and the ability to work as a part of a team and independently.
The successful candidates will receive an attractive salary in accordance with the MSCA regulations for Doctoral Researchers for a 3-year contract (full time employment). The salary includes a living allowance (gross amount: €1961,80 per month), plus a mobility allowance (€600 per month), and a family allowance (€650 per month if applicable). The precise (net) salary will be confirmed upon appointment.
In addition, such a monthly salary, all REBONE fellows will benefit from a wide range of training and mentoring activities, which includes secondments to be organised at the premises of the REBONE Beneficiaries and Associated Partners, as well as of a wide variety of training modules (related to both scientific and transferable skills).
Where to request additional information for this position:
Prof. Dr Bojana Obradovic, email: email@example.com
In order to complete the application procedure please prepare in advance the following documents:
- Application summary and Privacy consent (pdf file, please download the form HERE)
- CV using the European template (pdf file)
- List of publications (pdf file, free format)
- Copy of up to three selected publications, if available (zip file with three selected publications)
- Certificate of English language (pdf file)
- Signed copy of Identity card/Passport (pdf file)
- Motivation letters (pdf file)
- Two recommendation letters (zip file with two pdf)
- A research proposal: A document with no more than 8000 characters (excluding Bibliography) describing a potential research activity pertinent to the specific topic of the DC to be recruited. Figures and tables are allowed. Template provided. (pdf file, please download the template HERE)